A global fit can significantly improve the signal-to-noise ratio of multi-exponential analysis of FLIM data. In the past, global fitting has only rarely been used because the calculation times were unacceptably long. Now, the GPU processing used in BH’s SPCImage NG data analysis software has put the calculation times for global fitting in the range of a few 10 seconds to a few minutes. The reduced data processing times make the global fit attractive to a number of multi-exponential data analysis tasks in which the component lifetimes are unknown but invariable over the entire sample or over an area of interest. Please see application note, ‘Fast GPU-Based Global Fit of TCSPC FLIM Data‘. The figure below shows quantitative analysis of bound FAD, free FAD, and FMN in a live-cell sample. For download of new SPCImage NG software please see ‘SPCImage NG Data Analysis Software’. The downwload is free for existing SPCImage NG users.
